The BioDeviceLab Total Tau ELISA Kit is a high-performance sandwich enzyme-linked immunosorbent assay developed for the quantitative determination of total Tau protein in biological samples. Tau is a microtubule-associated protein abundantly expressed in neurons and serves as a widely used biomarker of neuroaxonal injury, neurodegeneration, and central nervous system pathology. The assay combines high sensitivity, robust reproducibility, and a wide dynamic detection range, making it suitable for both routine laboratory analysis and advanced biodevice development workflows.
Intended Use
This kit is intended for the quantitative determination of total Tau protein in:
• Serum
• Plasma
• Cerebrospinal fluid (CSF)
• Cell culture supernatants
Applications include Alzheimer’s disease research, neurodegeneration and neuroinflammation studies, traumatic brain injury and stroke research, organ-on-chip models, and biodevice validation and calibration.
Assay Principle
This assay employs a sandwich ELISA format:
• Total Tau present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody specific to a distinct Tau epitope binds to the captured protein.
• An enzyme conjugate is added, followed by a chromogenic substrate (TMB).
• The enzymatic reaction generates a color signal measured at 450 nm.
• Absorbance is directly proportional to the total Tau concentration in the sample.
Calibration Curve
• A calibration curve is generated using Total Tau standards with known concentrations.
• Mean absorbance values are plotted against Tau concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
• Sample concentrations are calculated by interpolation from the standard curve.
Analytical Performance
Dynamic Detection Range
• 15 – 2,000 pg/mL
Analytical Sensitivity
• Limit of Detection (LOD): ≤ 5 pg/mL
Precision
• Intra-assay CV: < 10%
• Inter-assay CV: < 10%
Selectivity & Specificity
The assay demonstrates high specificity for human Tau with minimal cross-reactivity to other microtubule-associated or cytoskeletal proteins. Representative cross-reactivity:
• Total Tau: 100%
• Phosphorylated Tau isoforms: detected as part of total Tau
• Neurofilament proteins, GFAP, α-synuclein: < 1%
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• Total Tau standards (lyophilized, multiple concentrations)
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Required Materials (Not Provided)
• Microplate reader capable of measuring absorbance at 450 nm
• Adjustable micropipettes and disposable tips
• Plate washer or manual washing equipment
• Plate shaker (recommended)
• Deionized or distilled water
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Avoid repeated freeze–thaw cycles.
• Samples exceeding the highest standard should be diluted using assay buffer.
• Hemolyzed or lipemic samples may affect assay accuracy.
Quality Control
• Standards and controls should be included in each assay run.
• Duplicate measurements are recommended.
• Acceptance criteria should be established by the user according to the intended application.
BioDeviceLab applies quality-controlled reagent preparation and batch-to-batch consistency practices to support reproducibility and scalability for biodevice development.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
• Opened reagents should be handled according to protocol recommendations.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic procedures.
• Results should be interpreted within the context of experimental design and sample matrix.
Applications
• Neurodegeneration and Alzheimer’s disease research
• Traumatic brain injury and stroke studies
• Neuroaxonal injury assessment
• Organ-on-chip and tissue models
• Biodevice calibration and benchmarking
Calibration Curve
