Product Description
The BioDeviceLab Human Mucin-2 (MUC2) ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed for the quantitative determination of MUC2 in biological samples. MUC2 is the dominant gel-forming mucin secreted by goblet cells and constitutes the primary structural component of the intestinal mucus layer.
The mucus layer plays a critical role in separating luminal microbes from the epithelial surface, regulating microbial colonization, and maintaining intestinal homeostasis. Alterations in MUC2 production, secretion, or structure are associated with inflammatory bowel disease, infection, dysbiosis, and barrier dysfunction. In gut-on-chip platforms, MUC2 serves as a key functional biomarker for evaluating mucus layer formation, goblet cell function, and microbial–epithelial interactions under physiological and pathological conditions. This assay combines high analytical sensitivity, strong specificity, and robust reproducibility, making it suitable for both mechanistic studies and advanced biodevice validation workflows.
Intended Use
This kit is intended for the quantitative determination of human MUC2 in:
• Cell culture supernatants
• Tissue lysates
• Fecal extracts
Applications include gut-on-chip mucus layer validation, goblet cell differentiation studies, host–microbiome interaction research, inflammatory bowel disease modeling, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format:
• MUC2 present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody specific to MUC2 binds to the captured protein.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to the MUC2 concentration in the sample.
Calibration Curve
• A calibration curve is generated using recombinant human MUC2 standards with known concentrations.
• Mean absorbance values are plotted against MUC2 concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
• Sample concentrations are calculated by interpolation from the standard curve.
Analytical Performance
Dynamic detection range: 0.1 – 40 ng/mL
Limit of detection (LOD): ≤ 0.05 ng/mL
Intra-assay CV: < 10%
Inter-assay CV: < 12%
Selectivity & Specificity
The assay demonstrates high specificity for MUC2 with minimal cross-reactivity to other mucins (MUC1, MUC4, MUC5AC) or unrelated glycoproteins (< 1%).
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• Recombinant human MUC2 standards
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Required Materials (Not Provided)
• Microplate reader capable of measuring absorbance at 450 nm
• Adjustable micropipettes and disposable tips
• Plate washer or manual washing equipment
• Plate shaker (recommended)
• Deionized or distilled water
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Avoid repeated freeze–thaw cycles.
• Samples exceeding the highest standard should be diluted using assay buffer.
• Highly viscous samples may require clarification prior to analysis.
Quality Control
• Standards and controls should be included in each assay run.
• Duplicate measurements are recommended.
• Acceptance criteria should be established according to experimental requirements.
BioDeviceLab applies quality-controlled reagent preparation and batch-to-batch consistency practices to support reproducibility and scalability for biodevice development.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
• Opened reagents should be handled according to protocol recommendations.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic procedures.
• Results should be interpreted within the context of experimental design and mucus-producing model.
Applications
• Gut-on-chip mucus layer integrity validation
• Goblet cell differentiation and function studies
• Host–microbiome interaction research
• Inflammatory bowel disease modeling
• Biodevice calibration and benchmarking
