Product Description
Vimentin is a ~54–57 kDa type III intermediate filament protein that plays a central role in maintaining cellular integrity, mechanical stability, and cytoskeletal organization. It is highly expressed in mesenchymal cells, fibroblasts, endothelial cells, and cells undergoing epithelial–mesenchymal transition. In contrast, mature epithelial cells typically show low or absent vimentin expression.
Within cells, vimentin forms an extensive filamentous network spanning the cytoplasm and connecting the nucleus to the cell periphery. Changes in vimentin expression, organization, or distribution are associated with cell migration, mechanical adaptation, differentiation, and phenotypic switching. As a result, vimentin is one of the most widely used markers for assessing mesenchymal identity and cellular plasticity.
The BioDeviceLab Vimentin Antibody is supplied as a purified monoclonal antibody to support flexible assay design. It can be used with secondary antibodies for indirect detection, adapted for custom labeling strategies, or integrated into biosensor, biodevice, and high-content analytical workflows. The antibody is compatible with conventional culture systems as well as microfluidic, organ-on-chip, and advanced in vitro platforms.
Intended Use
This product is intended for research applications including:
• Mesenchymal cell identification and characterization
• Epithelial–mesenchymal transition (EMT) analysis
• Immunofluorescence and immunocytochemistry imaging
• Organoid, spheroid, and 3D culture studies
• Cell migration and cytoskeletal organization studies
• Tissue engineering and regenerative medicine research
• Biosensor, biodevice, and assay biological validation
• Multiplex phenotyping and cell state analysis
Principle of Operation
Vimentin is an intracellular intermediate filament protein. Binding of the antibody following permeabilization enables
• Visualization of cytoskeletal filament networks by immunofluorescence
• Identification of mesenchymal or EMT-associated cell populations
• Assessment of cytoskeletal remodeling in response to mechanical or biochemical cues
• Integration of structural markers with signaling or functional readouts
Technical Information
Target antigen: Vimentin (VIM)
Alternative names: Intermediate filament protein vimentin
Protein type: Type III intermediate filament protein
Approximate molecular weight: ~54–57 kDa
Cellular localization: Cytoplasm (filamentous network)
Clonality: Monoclonal
Host species: Mouse
Isotype: IgG (clone dependent)
Commonly used commercial clones: V9, SP20, RV202
Validated applications (clone dependent): IF/ICC, IHC, western blot, flow cytometry
Typical stock concentration (industry reference): approximately 0.5 mg/mL
Reactivity: Human (other species dependent on clone validation)
Sample Volume and Typical Usage
Recommended sample volumes depend on assay format and platform and should be optimized experimentally. Common working ranges are summarized below.
Immunofluorescence / Immunocytochemistry
• Staining volume: 100 µL per sample
• Applicable to coverslips, chamber slides, well plates, tissue sections, and on-chip chambers
• Permeabilization is required for cytoplasmic filament access
Organoid and 3D Culture Staining
• Typical staining volume: 100–500 µL per sample
• Volume depends on matrix density and construct size
• Extended incubation times may be required for uniform penetration
Microfluidic and Biodevice Platforms
• Typical staining volume: 10–100 µL per device
• Volume depends on channel geometry and surface area
• Permeabilization conditions should be optimized for confined systems
General notes
• Antibody concentration is more critical than absolute volume
• Maintain consistent antibody-to-cell or antibody-to-surface ratios
• Optimization is recommended for each assay format and platform
Selectivity and Compatibility
Vimentin is selectively enriched in mesenchymal and EMT-associated cell states and is typically absent or low in fully differentiated epithelial cells. The antibody is compatible with standard fixation and permeabilization protocols and performs reliably across conventional culture systems, 3D models, and microengineered platforms.
Package Contents
Each unit contains
• Purified BioDeviceLab Vimentin (VIM) monoclonal antibody
• Lot-specific quality control documentation
Required Materials
• Fluorescent secondary antibody for unconjugated formats
• Fixation and permeabilization reagents
• Blocking and wash buffers
• Fluorescence microscope and/or flow cytometer
• Optional isotype and negative controls
Sample Handling and Use
• Store according to label instructions
• Avoid repeated freeze–thaw cycles
• Optimize staining conditions for each assay format
• Include appropriate controls for quantitative analyses
Quality Control
Each lot is evaluated for binding specificity and filamentous staining pattern. The antibody is designed to support reproducible mesenchymal and EMT profiling across diverse experimental systems.
Storage and Stability
• Store at 2–8 °C
• Do not freeze under standard storage conditions
• Protect from contamination and prolonged light exposure
• Proper storage preserves antibody stability and performance
Applications
• Mesenchymal and EMT marker analysis
• Cytoskeletal organization studies
• Tissue engineering and regenerative medicine
• Organoid and 3D culture systems
• Immunofluorescence-based phenotyping
• Biosensor and biodevice validation
• Cell plasticity and migration research
