Product Description
CD44 is a type I transmembrane cell-surface glycoprotein that functions as a principal receptor for hyaluronan and interacts with a broad range of extracellular matrix components, including collagen, osteopontin, and fibronectin. Through these interactions, CD44 plays a central role in cell adhesion, migration, mechanotransduction, and tissue remodeling.
CD44 exists as a standard isoform (CD44s) and multiple variant isoforms (CD44v) generated through alternative splicing. The relative expression of these isoforms is dynamically regulated during epithelial differentiation, epithelial–mesenchymal transition (EMT), wound healing, and cancer progression. Elevated CD44 expression is frequently associated with epithelial plasticity, stem-like or progenitor cell states, immune cell trafficking, and tumor invasion.
Immunostaining of CD44 reveals membrane-localized and pericellular labeling patterns that provide spatial insight into cell–cell and cell–matrix interactions. In 3D cultures and organoids, CD44 expression often highlights subpopulations with enhanced regenerative or invasive potential. The BioDeviceLab CD44 Antibody is supplied as a purified monoclonal antibody to support flexible assay design and integration across conventional and advanced in vitro platforms.
The antibody is compatible with standard immunostaining workflows and is well suited for use in 2D cultures, 3D organoids, spheroids, and organ-on-chip systems where epithelial heterogeneity, stemness, or EMT-related phenotypes are investigated.
Intended Use
This product is intended for research applications including
• Cell-surface marker analysis and phenotyping
• Epithelial plasticity and EMT studies
• Stem-like and progenitor cell identification
• Immunofluorescence and immunocytochemistry imaging
• Organoid, spheroid, and 3D culture characterization
• Organ-on-chip and microfluidic model validation
• Cancer biology and invasion studies
• Cell–matrix interaction analysis
• Biosensor, biodevice, and assay biological validation
Principle of Operation
CD44 is localized at the plasma membrane and pericellular matrix interface. Following appropriate fixation and, when required, permeabilization, antibody binding enables
• Visualization of CD44 membrane localization by immunofluorescence
• Identification of stem-like or EMT-associated subpopulations
• Assessment of cell–matrix interaction patterns
• Correlation of CD44 expression with epithelial plasticity or migration
• Integration of surface-marker readouts with cytoskeletal or ECM markers
Technical Information
Target antigen: CD44
Alternative names: HCAM, Hermes antigen
Protein type: Transmembrane cell-surface glycoprotein
Approximate molecular weight: ~85–200 kDa (isoform dependent)
Cellular localization: Plasma membrane, pericellular matrix
Clonality: Monoclonal
Host species: Mouse
Isotype: IgG (clone dependent)
Commonly used commercial clones: IM7, DF1485, G44-26
Validated applications (clone dependent): IF/ICC, IHC, flow cytometry, western blot
Typical stock concentration (industry reference): approximately 0.5 mg/mL
Reactivity: Human (other species dependent on clone validation)
Sample Volume and Typical Usage
Recommended sample volumes depend on assay format and platform and should be optimized experimentally. Common working ranges are summarized below.
Immunofluorescence / Immunocytochemistry
• Typical staining volume: 50–200 µL per sample
• Applicable to coverslips, chamber slides, well plates, tissue sections, and on-chip chambers
• Mild permeabilization may be used depending on epitope accessibility
Organoid and 3D Culture Staining
• Typical staining volume: 100–500 µL per sample
• Volume depends on construct size and matrix composition
• Extended incubation improves penetration and surface labeling
Microfluidic and Biodevice Platforms
• Typical staining volume: 10–100 µL per device
• Volume depends on channel geometry and chamber volume
• Flow-assisted staining may enhance uniform membrane labeling
General notes
• Antibody concentration is more critical than absolute volume
• Maintain consistent antibody-to-cell ratios
• Optimization is recommended for each assay format
Selectivity and Compatibility
CD44 is selectively enriched at the surface of epithelial, mesenchymal, immune, and stem-like cells depending on tissue context. The antibody is compatible with commonly used fixation and staining protocols and performs reliably across conventional culture systems, 3D models, and microengineered platforms.
Package Contents
Each unit contains
• Purified BioDeviceLab CD44 monoclonal antibody
• Lot-specific quality control documentation
Required Materials
• Fluorescent secondary antibody for unconjugated formats
• Fixation and optional permeabilization reagents
• Blocking and wash buffers
• Fluorescence microscope
• Optional isotype and negative controls
Sample Handling and Use
• Store according to label instructions
• Avoid repeated freeze–thaw cycles
• Optimize fixation to preserve surface epitopes
• Include appropriate controls for comparative analyses
Quality Control
Each lot is evaluated for binding specificity and membrane-associated staining performance. The antibody is designed to support reproducible analysis of cell-surface phenotypes across diverse experimental systems.
Storage and Stability
• Store at 2–8 °C
• Do not freeze under standard storage conditions
• Protect from contamination and prolonged light exposure
• Proper storage preserves antibody stability and performance
Applications
• Epithelial plasticity and EMT research
• Stem-like and progenitor cell studies
• Organoid and 3D culture systems
• Organ-on-chip and microfluidic models
• Cancer invasion and metastasis research
• Cell–matrix interaction studies
• Immunofluorescence-based surface marker phenotyping
• Biosensor and biodevice validation
