Product Overview
The BioDeviceLab Human LBP ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed for the quantitative determination of lipopolysaccharide-binding protein (LBP), an acute-phase protein primarily produced by hepatocytes and intestinal epithelial cells. LBP plays a central role in innate immune sensing by binding bacterial lipopolysaccharide (LPS) and facilitating its presentation to CD14/TLR4 signaling complexes.
In gastrointestinal systems, circulating and locally secreted LBP serves as a sensitive biomarker of microbial translocation, epithelial barrier disruption, and dysregulated host–microbiome interactions. Elevated LBP levels are associated with increased gut permeability, systemic inflammation, metabolic dysregulation, and inflammatory bowel disease. In gut-on-chip platforms, LBP provides a robust quantitative readout of endotoxin exposure, epithelial leakage, and immune activation under microbial challenge. This assay combines strong analytical sensitivity, high specificity, and excellent reproducibility, making it suitable for mechanistic studies and advanced biodevice validation workflows.
Intended Use
This kit is intended for the quantitative determination of LBP in:
• Serum
• Plasma
• Cell culture supernatants
Applications include gut-on-chip permeability and microbial translocation studies, host–microbiome interaction analysis, endotoxin challenge models, inflammatory signaling research, drug and biologic screening, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format:
• LBP present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody specific to LBP binds to the captured protein.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to the LBP concentration in the sample.
Calibration Curve
• A calibration curve is generated using recombinant human LBP standards with known concentrations.
• Mean absorbance values are plotted against LBP concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
• Sample concentrations are calculated by interpolation from the standard curve.
Analytical Performance
Dynamic Detection Range
• 0.5 – 50 µg/mL
Analytical Sensitivity
• Limit of Detection (LOD): ≤ 0.2 µg/mL
Precision
• Intra-assay CV: < 9%
• Inter-assay CV: < 7%
Selectivity & Specificity
The assay demonstrates high specificity for human LBP with minimal cross-reactivity to related acute-phase proteins (CRP, CD14, MD-2) (< 1%).
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• LBP standards (lyophilized, multiple concentrations)
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Avoid repeated freeze–thaw cycles.
• Samples exceeding the highest standard should be diluted using assay buffer.
• Clarify samples prior to analysis if turbidity is present.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
Applications
• Gut-on-chip permeability and microbial translocation studies
• Host–microbiome interaction analysis
• Endotoxin and LPS challenge models
• Inflammatory signaling research
• Drug and biologic screening
• Biodevice calibration and benchmarking
