Product Description
The BioDeviceLab Human Transforming Growth Factor Beta 1 (TGF-β1) ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed for the quantitative determination of TGF-β1 in biological samples. TGF-β1 is a pleiotropic cytokine that plays a central role in immune tolerance, epithelial differentiation, extracellular matrix remodeling, and tissue repair.
In the gastrointestinal tract, TGF-β1 is essential for maintaining mucosal immune homeostasis, regulating inflammatory responses, and promoting epithelial restitution following injury. Dysregulated TGF-β1 signaling is associated with chronic inflammation, fibrosis, impaired barrier recovery, and altered host–microbiome interactions. In gut-on-chip systems, TGF-β1 serves as a critical biomarker for studying immune suppression, epithelial healing, fibroblast activation, and long-term tissue remodeling. This assay combines high analytical sensitivity, strong specificity, and robust reproducibility, making it suitable for mechanistic immune regulation studies and advanced biodevice validation workflows.
Intended Use
This kit is intended for the quantitative determination of human TGF-β1 in:
• Serum
• Plasma
• Cell culture supernatants
• Tissue culture media
Applications include gut-on-chip immune regulation and epithelial repair studies, fibrosis and tissue remodeling research, host–microbiome tolerance modeling, drug and biologic screening, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format with sample activation:
• Latent TGF-β1 in samples is first activated according to the provided protocol.
• Activated TGF-β1 binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody specific to TGF-β1 binds to the captured cytokine.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to the TGF-β1 concentration in the sample.
Calibration Curve
• A calibration curve is generated using recombinant human TGF-β1 standards with known concentrations.
• Mean absorbance values are plotted against TGF-β1 concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
• Sample concentrations are calculated by interpolation from the standard curve.
Analytical Performance
Dynamic detection range: 15 – 2,000 pg/mL
Limit of detection (LOD): ≤ 5 pg/mL
Intra-assay CV: < 10%
Inter-assay CV: < 12%
Selectivity & Specificity
The assay demonstrates high specificity for TGF-β1 with minimal cross-reactivity to other TGF-β isoforms (TGF-β2, TGF-β3) and cytokines (< 1%).
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• Recombinant human TGF-β1 standards
• Sample activation reagents
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Required Materials (Not Provided)
• Microplate reader capable of measuring absorbance at 450 nm
• Adjustable micropipettes and disposable tips
• Plate washer or manual washing equipment
• Plate shaker (recommended)
• Deionized or distilled water
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Perform sample activation as specified in the protocol prior to analysis.
• Avoid repeated freeze–thaw cycles.
• Samples exceeding the highest standard should be diluted using assay buffer.
Quality Control
• Standards and controls should be included in each assay run.
• Duplicate measurements are recommended.
• Acceptance criteria should be established according to experimental requirements.
BioDeviceLab applies quality-controlled reagent preparation and batch-to-batch consistency practices to support reproducibility and scalability for biodevice development.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
• Opened reagents should be handled according to protocol recommendations.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic procedures.
• Results should be interpreted within the context of experimental design and tissue remodeling models.
Applications
• Gut-on-chip immune tolerance and epithelial repair studies
• Fibrosis and extracellular matrix remodeling research
• Host–microbiome immune regulation
• Drug and biologic screening
• Biodevice calibration and benchmarking
