Product Description
The BioDeviceLab Myxovirus Resistance Protein A (MxA) ELISA Kit is a high-performance sandwich enzyme-linked immunosorbent assay developed for the quantitative determination of human MxA in biological samples. MxA is an interferon-induced dynamin-like GTPase and a well-established biomarker of type I interferon (IFN-α/β) activity. Its expression is strongly upregulated during acute viral infections and interferon-driven immune responses, while remaining low in most bacterial infections.
Because of this host-response specificity, MxA is widely used to differentiate viral from bacterial infections, to monitor antiviral immune activation, and to study interferon pathway dynamics. This assay combines high analytical sensitivity, excellent specificity, and robust reproducibility, enabling reliable MxA quantification for both conventional laboratory research and advanced biodevice validation workflows.
For Research Use Only (RUO).
Intended Use
This kit is intended for the quantitative determination of MxA in:
• Serum
• Plasma
• Whole blood or PBMC lysates
• Cell culture supernatants
Applications include viral infection research, host immune response profiling, interferon signaling studies, point-of-care assay development, organ-on-chip immune models, and biodevice validation and calibration.
Assay Principle
This assay employs a sandwich ELISA format:
• MxA present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody specific to MxA binds to the captured protein.
• An enzyme conjugate is added, followed by a chromogenic substrate (TMB).
• The enzymatic reaction generates a color signal measured at 450 nm.
• Absorbance is directly proportional to the MxA concentration in the sample.
Calibration Curve
• A calibration curve is generated using MxA standards with known concentrations.
• Mean absorbance values are plotted against MxA concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
Analytical Performance
Dynamic detection range: 0.2 – 20 ng/mL
Limit of detection (LOD): ≤ 0.05 ng/mL
Intra-assay CV: < 10%
Inter-assay CV: < 12%
(Performance aligned with commercial MxA ELISA kits used in viral diagnostics and interferon research.)
Selectivity & Specificity
The assay demonstrates high specificity for human MxA with minimal cross-reactivity to other interferon-stimulated or antiviral proteins. Representative cross-reactivity levels:
• MxA: 100%
• MxB, ISG15, OAS1, IFIT family proteins: < 1%
This specificity enables reliable assessment of interferon-driven antiviral responses.
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• MxA standards (lyophilized, multiple concentrations)
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Required Materials (Not Provided)
• Microplate reader capable of measuring absorbance at 450 nm
• Adjustable micropipettes and disposable tips
• Plate washer or manual washing equipment
• Plate shaker (recommended)
• Deionized or distilled water
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Avoid repeated freeze–thaw cycles.
• Whole blood or PBMC lysates should be clarified before analysis.
• Samples exceeding the highest standard should be diluted using assay buffer.
Quality Control
• Standards and controls should be included in each assay run.
• Duplicate measurements are recommended.
• Acceptance criteria should be established by the user according to the intended application.
BioDeviceLab applies quality-controlled reagent preparation and batch-to-batch consistency practices to support reproducibility and scalability for biodevice development.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
• Opened reagents should be handled according to protocol recommendations.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic procedures.
• Results should be interpreted within the context of experimental design and immune status.
Applications
• Viral vs bacterial infection differentiation
• Type I interferon signaling studies
• Antiviral immune response profiling
• Organ-on-chip and immune-on-chip models
• Biodevice calibration and benchmarking
