Product Description
The BioDeviceLab 20 nm Gold Nanoparticles are precision-synthesized colloidal particles that exhibit strong localized surface plasmon resonance (LSPR) and narrow size distributions (<15% dispersity). At ~20 nm diameter, these nanoparticles provide enhanced optical absorption and scattering properties that are advantageous for UV–Vis–based detection, colorimetric assays, and plasmonic biosensing.
Formulated with citrate/tannic acid stabilization in aqueous buffer, the 20 nm gold nanoparticles are compatible with biological assays, microfluidic systems, and lab-on-chip platforms. The characteristic SPR absorbance peak near 520–525 nm enables reproducible optical and spectroscopic characterization, while the particle surface facilitates efficient passive adsorption of proteins and ligands for conjugation workflows without requiring additional surface modification.
The 500 mL bulk volume supports scalable assay development, conjugate production, and repeated use across iterative experiments. These nanoparticles are suitable for analytical workflows, biosensor calibration, and signal enhancement in a variety of applications.
Intended Use
This product is intended for research applications including:
• Biosensor and plasmonic assay development
• Protein, antibody, and ligand conjugation (passive adsorption)
• Optical and colorimetric signal amplification
• Microfluidic and lab-on-chip integration
• Biodevice calibration and benchmarking
• Imaging contrast and analytical probing
Principle of Operation
Gold nanoparticles exhibit localized surface plasmon resonance (LSPR), where conduction electrons on the particle surface oscillate collectively in response to incident light.
• The SPR peak position and intensity depend on particle characteristics and environment.
• Biomolecule binding events or changes in local refractive index alter the SPR signal for optical sensing.
• High surface area at 20 nm supports effective biomolecule interaction and functionalization.
• These properties make 20 nm gold nanoparticles effective reporters and signal amplifiers in biosensing and analytical platforms.
Calibration & Optical Characterization
• Optical density (OD) is defined at the SPR peak using a 1 cm pathlength.
• UV–Vis spectroscopy is used to verify the characteristic absorbance peak (~520–525 nm).
• Molar extinction coefficients and particle concentration are calculated based on OD = 1.
• Dynamic Light Scattering (DLS) confirms hydrodynamic size and narrow dispersity.
• Optical and size metrics should be confirmed for each experimental design.
Analytical & Physical Characteristics
Nominal particle diameter: ~20 nm
Peak SPR wavelength: ~520–525 nm
Particle concentration: ~1.07 × 10¹³ particles/mL
Weight concentration: ~5.25 × 10⁻¹ mg/mL
Molar extinction coefficient: ~4.05 × 10⁷ M⁻¹·cm⁻¹
Size dispersity: <15%
Surface area per particle: ~1.26 × 10³ nm²
Surface-to-volume ratio: ~0.3
Particle mass: ~3.63 × 10⁻¹⁶ g
Molar mass: ~5.44 × 10⁷ g/mol
Molar concentration: ~1.97 × 10⁻⁹ M
Supplied volume: 500 mL
Selectivity & Compatibility
Gold nanoparticles are chemically inert and broadly compatible with biomolecules and assay platforms. They can be integrated with proteins, nucleic acids, polymers, and electrochemical interfaces without interfering with biological recognition or signal transduction.
Package Contents
Each unit contains:
• 500 mL of 20 nm citrate-stabilized gold nanoparticle colloidal suspension
• Optical characterization and quality control documentation
Required Materials (Not Provided)
• UV–Vis spectrophotometer
• Dynamic Light Scattering (DLS) instrument (recommended)
• Standard laboratory pipettes and consumables
• Buffers compatible with downstream functionalization
Sample Handling & Use
• Gently swirl before use to achieve uniform suspension.
• Avoid vigorous shaking or sonication that may cause aggregation.
• Dilute with compatible aqueous buffers for downstream applications.
• Minimize repeated contamination of the stock solution.
Quality Control
• Each production lot is verified for SPR peak position, particle concentration, and size distribution.
• Batch consistency supports reproducibility in biodevice development workflows.
• Optical density and size metrics follow standardized QC practices.
Storage & Stability
• Store at 4–25 °C.
• Do not freeze.
• Protect from prolonged direct light exposure.
• Proper storage maintains colloidal stability and performance throughout the shelf life.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic use.
• Performance may vary with buffer conditions and downstream functionalization strategies.
Applications
• Biosensor and plasmonic device development
• Optical and colorimetric assay design
• Protein and antibody conjugate preparation
• Microfluidic and lab-on-chip systems
• Biodevice calibration and benchmarking
• Imaging contrast and analytical probing
