Product Description
The BioDeviceLab 5 nm Gold Nanoparticles are precision-engineered nanomaterials that exploit size-dependent optical and surface properties arising from localized surface plasmon resonance (LSPR). At the 5 nm scale, gold nanoparticles exhibit a high surface-to-volume ratio, enhanced biomolecule adsorption, and excellent colloidal stability, making them particularly suitable for biosensing, conjugation, and signal amplification applications.
These nanoparticles are synthesized using controlled reduction chemistry to produce highly spherical particles with narrow size distributions (<15% dispersity) and reproducible batch-to-batch optical signatures. The particles are stabilized with citrate/tannic acid ligands and formulated in an aqueous buffered system compatible with biological samples, microfluidic devices, and lab-on-chip platforms.
The nanoparticles exhibit a characteristic SPR absorbance peak at 515–520 nm, enabling straightforward UV–Vis–based quality control, colorimetric assay development, and plasmonic sensing applications. The 500 mL bulk volume supports large-scale conjugate preparation, assay development, and repeated experimental use without lot-to-lot variability, aligning with biodevice validation and translational research needs.
Intended Use
This product is intended for research applications including:
• Biosensor and plasmonic assay development
• Antibody, protein, and ligand conjugation (passive adsorption)
• Optical and electrochemical signal amplification
• Microfluidic and lab-on-chip integration
• Assay calibration and benchmarking
• Imaging and contrast enhancement studies
Principle of Operation
Gold nanoparticles exhibit localized surface plasmon resonance, where collective oscillation of surface electrons occurs in response to incident light.
• The SPR peak position and intensity depend on particle size, shape, and local environment.
• Binding of biomolecules or changes in the surrounding medium alter the SPR signal, enabling optical sensing.
• High surface area facilitates efficient biomolecule adsorption and functionalization.
• These properties make gold nanoparticles effective transducers and signal enhancers in biosensing platforms.
Calibration & Optical Characterization
• Optical density (OD) is defined at the SPR peak wavelength using a 1 cm pathlength.
• UV–Vis spectroscopy is used to verify the characteristic SPR peak at 515–520 nm.
• Particle concentration, molar extinction coefficient, and molar concentration are calculated based on OD = 1.
• Dynamic light scattering (DLS) is used to confirm size distribution and colloidal stability.
• Optical and size characterization should be verified for each experimental workflow.
Analytical & Physical Characteristics
Nominal particle diameter: 5 nm
Peak SPR wavelength: 515–520 nm
Particle concentration: 5.47 × 10¹³ particles/mL
Weight concentration: 6.94 × 10⁻² mg/mL
Molar extinction coefficient: 1.10 × 10⁷ M⁻¹·cm⁻¹
Size dispersity: <15%
Particle volume: 6.54 × 10¹ nm³
Particle surface area: 7.85 × 10¹ nm²
Surface-to-volume ratio: 1.2
Particle mass: 1.27 × 10⁻¹⁸ g
Molar mass: 7.64 × 10⁵ g/mol
Molar concentration: 9.08 × 10⁻⁸ M
Supplied volume: 500 mL
Selectivity & Compatibility
Gold nanoparticles are chemically inert and exhibit broad compatibility with biological systems. They show no intrinsic cross-reactivity and can be readily integrated with proteins, antibodies, nucleic acids, polymers, and electrochemical interfaces without interfering with biological recognition processes.
Package Contents
Each unit contains:
• 500 mL of 5 nm citrate-stabilized gold nanoparticle colloidal suspension
• Quality control documentation including optical characterization specifications
Required Materials (Not Provided)
• UV–Vis spectrophotometer
• Dynamic light scattering (DLS) instrument (recommended)
• Standard laboratory glassware and pipettes
• Buffer solutions appropriate for downstream functionalization
Sample Handling & Use
• Gently swirl before use to ensure uniform suspension.
• Avoid vigorous shaking or sonication that may induce aggregation.
• Dilute using compatible aqueous buffers if required.
• Avoid repeated contamination of the stock solution.
Quality Control
• Each production lot is characterized for SPR peak position, particle concentration, and size distribution.
• Batch-to-batch consistency is maintained to support reproducibility in biodevice development and assay scaling.
• Optical density and size metrics are traceable to standardized characterization procedures.
Storage & Stability
• Store at 4–25 °C.
• Do not freeze.
• Protect from prolonged exposure to direct light.
• When stored properly, the colloid maintains stability and optical performance throughout its shelf life.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic use.
• Performance may vary depending on buffer composition and downstream functionalization strategy.
Applications
• Biosensor and plasmonic device development
• Colorimetric and optical assay design
• Antibody and protein conjugate preparation
• Microfluidic and lab-on-chip systems
• Biodevice calibration and benchmarking
• Imaging and contrast enhancement studies
