Product Description
The BioDeviceLab 100 nm Gold Nanoparticles are precision-synthesized colloidal gold particles that provide a pronounced localized surface plasmon resonance (LSPR) response and broad applicability in biological and engineering systems. At ~100 nm diameter, these nanoparticles exhibit enhanced light scattering, strong optical cross sections, and a characteristic plasmon peak at longer visible wavelengths compared with smaller particles, expanding their utility in imaging, plasmonic sensing, and signal amplification applications.
These nanoparticles are stabilized with citrate/tannic acid ligands and formulated in aqueous buffer to ensure compatibility with biological assays, microfluidic devices, and lab-on-chip platforms. The 500 mL bulk supply supports large-scale conjugate preparations, reproducible assay development, and repeated experimental use. The high optical cross section and robust surface area make 100 nm gold nanoparticles particularly suitable for lateral flow development, enhanced colorimetric readouts, and optical contrast applications.
Intended Use
This product is intended for research applications including:
• Optical and plasmonic biosensor development
• Enhanced colorimetric and light-scattering assays
• Passive conjugation of proteins, antibodies, and ligands
• Imaging and contrast enhancement
• Microfluidic and lab-on-chip integration
• Biodevice calibration and benchmarking
Principle of Operation
Gold nanoparticles exhibit localized surface plasmon resonance (LSPR), whereby conduction electrons on the particle surface oscillate collectively in response to incident light.
• The SPR peak position and intensity are size-dependent and influenced by the local refractive index.
• Larger particles (such as 100 nm) produce stronger scattering and absorbance signals.
• Biomolecule binding or environmental changes modulate optical properties, enabling sensing.
• High surface area supports enhanced adsorption and interaction with biomolecules.
Calibration & Optical Characterization
• Optical density (OD) is defined at the SPR peak using a 1 cm pathlength.
• UV–Vis spectroscopy is used to verify the characteristic absorbance and scattering profiles.
• Molar extinction coefficients and particle concentrations are derived based on OD = 1.
• Dynamic Light Scattering (DLS) confirms hydrodynamic size and dispersity.
• Optical and size characterization should be verified for each experimental design.
Analytical & Physical Characteristics
Nominal particle diameter: ~100 nm
Peak SPR wavelength: ~525–540 nm (size-dependent)
Particle concentration: ~2.50 × 10¹¹ particles/mL
Weight concentration: ~2.50 × 10⁰ mg/mL
Molar extinction coefficient: ~1.00 × 10⁹ M⁻¹·cm⁻¹
Size dispersity: <15%
Surface area per particle: ~3.14 × 10⁴ nm²
Surface-to-volume ratio: ~0.2
Particle mass: ~3.27 × 10⁻¹⁴ g
Molar mass: ~4.90 × 10⁹ g/mol
Molar concentration: ~5.10 × 10⁻¹¹ M
Supplied volume: 500 mL
Selectivity & Compatibility
Gold nanoparticles are chemically inert and broadly compatible with biomolecules and assay platforms. They are suitable for integration with proteins, nucleic acids, polymers, and electrochemical interfaces without interfering with biological recognition.
Package Contents
Each unit contains:
• 500 mL of 100 nm citrate-stabilized gold nanoparticle colloidal suspension
• Optical characterization and quality control documentation
Required Materials (Not Provided)
• UV–Vis spectrophotometer
• DLS instrument (recommended)
• Standard laboratory glassware and pipettes
• Buffers compatible with downstream applications
Sample Handling & Use
• Swirl gently before use to ensure uniform suspension.
• Avoid vigorous agitation or sonication that may induce aggregation.
• Dilute with compatible aqueous buffers for downstream applications.
• Minimize repeated contamination of the stock solution.
Quality Control
• Each production lot is verified for SPR peak characteristics, particle concentration, and size distribution.
• Batch consistency supports reproducibility in biodevice development workflows.
• Optical and size metrics follow standardized QC practices.
Storage & Stability
• Store at 4–25 °C.
• Do not freeze.
• Protect from prolonged direct light exposure.
• Proper storage maintains colloidal stability and performance throughout the shelf life.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic use.
• Performance may vary with buffer conditions and functionalization strategies.
Applications
• Optical and plasmonic biosensor development
• Enhanced light-scattering and colorimetric assays
• Protein and antibody conjugate preparation
• Imaging and contrast enhancement
• Microfluidic and lab-on-chip systems
• Biodevice calibration and benchmarking
