Donkey Anti-Rabbit IgG (H+L) Alexa Fluor™ 488 is an affinity-purified polyclonal secondary antibody raised in donkey and covalently conjugated to Alexa Fluor™ 488. The antibody recognizes both heavy and light chains of rabbit IgG, providing strong and uniform signal amplification in indirect detection assays. Alexa Fluor™ 488 is a bright, photostable green fluorophore with high quantum yield and low background, making it well suited for multiplex fluorescence imaging and quantitative analysis. This secondary antibody is compatible with standard biological samples as well as advanced platforms including organoids, microfluidic systems, and organ-on-chip devices.
Intended Use
This product is intended for research applications including
• Immunofluorescence and immunocytochemistry
• Cell and tissue imaging
• Organoid, spheroid, and 3D culture analysis
• Multiplex fluorescence assays
• Microfluidic and organ-on-chip staining workflows
• Biosensor and biodevice validation
Principle of Operation
The antibody binds specifically to rabbit IgG molecules previously bound to target antigens by primary antibodies. Fluorescent labeling enables
• Indirect signal amplification
• High-sensitivity detection of antigen–antibody complexes
• Compatibility with multiplex fluorescence panels
• Stable signal generation under standard imaging conditions
Technical Information
Target: Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Isotype: IgG
Fluorophore: Alexa Fluor™ 488
Excitation maximum: ~495 nm
Emission maximum: ~519 nm
Conjugation method: Covalent fluorophore conjugation
Formulation: Aqueous buffered solution
Reactivity: Rabbit
Sample Volume and Typical Usage
Immunofluorescence and immunocytochemistry
• Typical staining volume: 50–200 µL per sample
• Suitable for coverslips, chamber slides, well plates, tissue sections, and on-chip chambers
Organoids and 3D cultures
• Typical staining volume: 100–500 µL per sample
• Volume depends on sample size and matrix density
Microfluidic and biodevice platforms
• Typical staining volume: 10–50 µL per device
• Concentration optimization recommended for confined geometries
General notes
• Optimize antibody dilution for each application
• Maintain consistent antibody-to-sample ratios
• Use appropriate negative and control samples
Selectivity and Compatibility
This secondary antibody exhibits strong selectivity for rabbit IgG with minimal cross-reactivity to other species. It is compatible with common fixation methods, blocking reagents, and buffer systems and performs reliably in conventional and device-integrated workflows.
Package Contents
Each unit contains
• BioDeviceLab Donkey Anti-Rabbit IgG (H+L) Alexa Fluor™ 488 Secondary Antibody
• Lot-specific quality control documentation
Storage and Stability
Store at 2–8 °C
Do not freeze
Protect from prolonged light exposure
Applications
• Fluorescence-based imaging assays
• Multiplex immunostaining
• Cell and tissue phenotyping
• Organoid and 3D culture analysis
• Microfluidic and organ-on-chip validation
• Biosensing and biodevice development
