Product Description
Ki-67, also known as MKI67, is a large nuclear protein with an approximate molecular weight of 320–360 kDa that is tightly associated with cellular proliferation. Ki-67 is expressed during all active phases of the cell cycle (G1, S, G2, and M phases) and is absent in quiescent (G0) cells, making it one of the most reliable markers for identifying dividing cell populations.
Because Ki-67 localizes to the nucleus and exhibits dynamic redistribution during mitosis, it is extensively used to evaluate proliferation rates, growth dynamics, and cell cycle activity in both normal and diseased systems. Ki-67 staining provides quantitative and spatial information on proliferative heterogeneity within cell populations, tissues, and engineered models.
The BioDeviceLab Ki-67 Antibody is supplied as a purified monoclonal antibody to support flexible assay design. It can be used with secondary antibodies for indirect detection, adapted for custom labeling strategies, or integrated into biosensor, biodevice, and high-content analysis workflows. The antibody is compatible with conventional culture systems as well as microfluidic, organ-on-chip, and advanced in vitro platforms.
Intended Use
This product is intended for research applications including
• Detection and quantification of proliferating cells
• Cell cycle and growth dynamics analysis
• Immunofluorescence and immunocytochemistry imaging
• Organoid, spheroid, and 3D culture studies
• Tissue engineering and regenerative medicine research
• Cancer and translational biology studies
• Biosensor, biodevice, and assay biological validation
• High-content and multiplex phenotyping assays
Principle of Operation
Ki-67 is expressed exclusively in actively cycling cells. Binding of the antibody to nuclear Ki-67 enables
• Identification of proliferating cells by immunofluorescence or microscopy
• Quantification of proliferation indices across cell populations
• Spatial mapping of growth patterns in 2D, 3D, and device-based systems
• Integration of proliferation metrics with structural or functional markers
Technical Information
Target antigen: Ki-67 (MKI67), proliferation marker
Alternative names: MKI67, KIA
Protein type: Nuclear proliferation-associated protein
Approximate molecular weight: ~320–360 kDa
Cellular localization: Nucleus (cell-cycle dependent distribution)
Clonality: Monoclonal
Host species: Mouse
Isotype: IgG (clone dependent)
Commonly used commercial clones: MIB-1, SP6, Ki-S5
Validated applications (clone dependent): IF/ICC, IHC, flow cytometry, western blot
Typical stock concentration (industry reference): approximately 0.5 mg/mL
Reactivity: Human (other species dependent on clone validation)
Sample Volume and Typical Usage
Recommended sample volumes depend on assay format and platform and should be optimized experimentally. Common working ranges are summarized below.
Immunofluorescence / Immunocytochemistry
• Typical staining volume: 50–200 µL per sample
• Applicable to coverslips, chamber slides, well plates, tissue sections, and on-chip chambers
• Nuclear permeabilization is required for antibody access
Flow Cytometry
• Typical staining volume: 50–100 µL per test
• Typical cell input: approximately 1 × 10⁵ to 1 × 10⁶ cells per test
• Fixation and permeabilization are required prior to staining
Organoid and 3D Culture Staining
• Typical staining volume: 100–500 µL per sample
• Volume depends on matrix density and construct size
• Extended incubation times may be required for nuclear penetration
Microfluidic and Biodevice Platforms
• Typical staining volume: 10–100 µL per device
• Volume depends on channel geometry and surface area
• Permeabilization conditions should be optimized for confined systems
General notes
• Antibody concentration is more critical than absolute volume
• Maintain consistent antibody-to-cell ratios
• Optimization is recommended for each platform
Selectivity and Compatibility
Ki-67 expression is restricted to actively dividing cells, enabling clear discrimination between proliferating and quiescent populations. The antibody is compatible with standard fixation, permeabilization, and staining protocols and performs reliably across conventional culture systems, 3D models, and microengineered platforms.
Package Contents
Each unit contains
• Purified BioDeviceLab Ki-67 monoclonal antibody
• Lot-specific quality control documentation
Required Materials
• Fluorescent secondary antibody for unconjugated formats
• Fixation and permeabilization reagents
• Blocking and wash buffers
• Fluorescence microscope and/or flow cytometer
• Optional isotype and negative controls
Sample Handling and Use
• Store according to label instructions
• Avoid repeated freeze–thaw cycles
• Optimize staining conditions for each assay format
• Include appropriate controls for quantitative analyses
Quality Control
Each lot is evaluated for binding specificity and signal-to-background performance. The antibody is designed to support reproducible proliferation analysis across diverse experimental systems and longitudinal studies.
Storage and Stability
• Store at 2–8 °C
• Do not freeze under standard storage conditions
• Protect from contamination and prolonged light exposure
• Proper storage preserves antibody stability and performance
Applications
• Cell proliferation and growth analysis
• Cancer and translational research
• Tissue engineering and regenerative medicine
• Organoid and 3D culture systems
• Flow cytometry and imaging assays
• Biosensor and biodevice validation
• Cell cycle and phenotyping studies
