Product Description
E-cadherin, also known as Cadherin-1 (CDH1), is a calcium-dependent transmembrane adhesion protein with an approximate molecular weight of 120 kDa. It is a core component of adherens junctions and plays a central role in epithelial tissue architecture by mediating homophilic cell–cell interactions. E-cadherin is essential for maintaining epithelial cohesion, polarity, and barrier function and is widely used as a canonical marker of epithelial identity.
E-cadherin is localized to the plasma membrane at sites of cell–cell contact, where it interacts with catenins and the actin cytoskeleton to form stable adhesion complexes. Antibody labeling produces a characteristic membrane-associated junctional pattern that provides a sensitive readout of epithelial organization, junctional continuity, and cellular differentiation state.
The BioDeviceLab E-cadherin Antibody is supplied as a purified monoclonal antibody to support flexible assay design. It can be used with secondary antibodies for indirect detection, adapted for custom conjugation strategies, or integrated into biosensor, biodevice, and cell-surface recognition workflows. The antibody is broadly applicable across epithelial biology, cancer research, tissue engineering, stem cell studies, and translational platform development.
Intended Use
This product is intended for research applications including
• Epithelial cell identification and phenotyping
• Visualization of adherens junctions and cell–cell adhesion
• Immunofluorescence and immunocytochemistry imaging
• Flow cytometric analysis of epithelial populations
• Organoid, spheroid, and 3D culture characterization
• Cell population analysis in mixed or co-culture systems
• Tissue engineering and regenerative medicine research
• Biosensor, biodevice, and assay biological validation
Principle of Operation
E-cadherin (CDH1) is expressed on the surface of epithelial cells at adherens junctions. Binding of the antibody to the extracellular domain enables
• Spatial visualization of epithelial cell–cell junctions by immunofluorescence
• Quantitative detection of E-cadherin-positive populations by flow cytometry
• Integration into multiplex assays linking adhesion status with polarity, differentiation, or signaling markers
Technical Information
Target antigen: E-cadherin (CDH1), Cadherin-1
Alternative names: CD324, ECAD, Uvomorulin
Protein type: Calcium-dependent transmembrane adhesion protein
Approximate molecular weight: ~120 kDa
Cellular localization: Cell surface, adherens junctions
Clonality: Monoclonal
Host species: Mouse
Isotype: IgG (clone dependent)
Commonly used commercial clones: HECD-1, 36/E-cadherin, DECMA-1, NCH-38
Validated applications (clone dependent): IF/ICC, flow cytometry, immunohistochemistry, western blot
Typical stock concentration (industry reference): approximately 0.5 mg/mL
Reactivity: Human (other species dependent on clone validation)
Sample Volume and Typical Usage
Recommended sample volumes depend on assay format and platform and should be optimized experimentally. Common working ranges are summarized below.
Immunofluorescence / Immunocytochemistry
• Typical staining volume: 50–200 µL per sample
• Applicable to coverslips, chamber slides, well plates, tissue sections, and on-chip staining chambers
• Volume should fully cover the cell or tissue surface to ensure uniform antibody access
Flow Cytometry
• Typical staining volume: 50–100 µL per test
• Typical cell input: approximately 1 × 10⁵ to 1 × 10⁶ cells per test
• Antibody amount is typically defined per test and should be titrated for each cell type
Organoid and 3D Culture Staining
• Typical staining volume: 100–500 µL per sample
• Volume depends on matrix composition, organoid density, and vessel geometry
• Extended incubation times may be required to ensure antibody penetration
Microfluidic and Biodevice Platforms
• Typical staining volume: 10–100 µL per device
• Volume depends on channel dimensions, surface area, and flow configuration
• Antibody concentration may require adjustment in confined or high-surface-area systems
General notes
• Antibody concentration is more critical than absolute volume
• Maintain consistent antibody-to-cell or antibody-to-surface ratios
• Optimization is recommended for each assay format and platform
Selectivity and Compatibility
E-cadherin is selectively expressed in epithelial cells and is generally absent or reduced in mesenchymal populations, enabling effective discrimination in heterogeneous samples. The antibody is compatible with standard fixation, staining, and buffering conditions and performs reliably in conventional culture systems as well as microfluidic, organ-on-chip, and biodevice-integrated workflows.
Package Contents
Each unit contains
• Purified BioDeviceLab E-cadherin (CDH1) monoclonal antibody
• Lot-specific quality control documentation
Required Materials
• Fluorescent secondary antibody for unconjugated formats
• Blocking and wash buffers compatible with IF or flow cytometry
• Fluorescence microscope and/or flow cytometer
• Optional isotype and negative controls
Sample Handling and Use
• Store according to label instructions
• Avoid repeated freeze–thaw cycles
• Optimize antibody concentration, volume, and incubation time for each assay format
• Use appropriate controls for quantitative and comparative analyses
Quality Control
Each lot is evaluated for binding specificity and membrane-associated staining performance. The antibody is designed to support reproducible epithelial adhesion profiling across diverse experimental platforms and longitudinal studies.
Storage and Stability
• Store at 2–8 °C
• Do not freeze under standard storage conditions
• Protect from contamination and prolonged light exposure
• Proper storage preserves antibody stability and performance
Applications
• Epithelial adhesion and junctional biology
• Cancer and translational research
• Stem cell differentiation studies
• Tissue engineering and regenerative medicine
• Organoid and 3D culture systems
• Flow cytometry and imaging-based assays
• Biosensing and biodevice validation
• Cell-surface marker profiling and enrichment studies
