Product Description
The BioDeviceLab 50 nm Stabilized Gold Nanoparticles are precision-synthesized colloidal gold particles that offer a balance between surface area and optical cross-section. At ~50 nm diameter, these nanoparticles exhibit pronounced localized surface plasmon resonance (LSPR), enhanced light scattering, and excellent colloidal stability, making them well suited for optical sensing, plasmonic signal amplification, and conjugation-based assays.
These nanoparticles are stabilized using citrate/tannic acid ligands in an aqueous formulation compatible with biological samples, microfluidic platforms, and lab-on-chip systems. The 50 nm size provides increased optical intensity compared to smaller nanoparticles while maintaining sufficient surface area for efficient passive adsorption of proteins, antibodies, and ligands.
The characteristic SPR absorbance peak near 525–535 nm enables reliable UV–Vis characterization and strong colorimetric responses. The 500 mL bulk format supports scalable assay development, conjugate preparation, and repeated experimental use with consistent performance across biodevice validation and translational research workflows.
Intended Use
This product is intended for research applications including:
• Biosensor and plasmonic assay development
• Optical and colorimetric signal amplification
• Passive conjugation of proteins, antibodies, and ligands
• Microfluidic and lab-on-chip integration
• Biodevice calibration and benchmarking
• Imaging and analytical contrast enhancement
Principle of Operation
Gold nanoparticles exhibit localized surface plasmon resonance (LSPR), resulting from collective oscillation of conduction electrons in response to incident light.
• The SPR peak position and intensity depend on particle size, shape, and surrounding medium.
• 50 nm particles provide enhanced scattering and absorbance compared to smaller sizes.
• Biomolecule binding events alter the local refractive index, modulating optical signals.
• These properties make 50 nm gold nanoparticles effective optical reporters and signal amplifiers.
Calibration & Optical Characterization
• Optical density (OD) is defined at the SPR peak wavelength using a 1 cm pathlength.
• UV–Vis spectroscopy is used to verify the characteristic absorbance peak (~525–535 nm).
• Particle concentration and molar extinction coefficients are calculated at OD = 1.
• Dynamic Light Scattering (DLS) confirms hydrodynamic size and dispersity.
• Optical and size metrics should be verified for each experimental workflow.
Analytical & Physical Characteristics
Nominal particle diameter: ~50 nm
Peak SPR wavelength: ~525–535 nm
Particle concentration: ~2.0 × 10¹² particles/mL
Weight concentration: ~1.0 mg/mL
Molar extinction coefficient: ~3.0 × 10⁸ M⁻¹·cm⁻¹
Size dispersity: <15%
Surface area per particle: ~7.85 × 10³ nm²
Surface-to-volume ratio: ~0.24
Particle mass: ~1.02 × 10⁻¹⁵ g
Molar mass: ~6.15 × 10⁸ g/mol
Molar concentration: ~3.2 × 10⁻¹⁰ M
Supplied volume: 500 mL
Selectivity & Compatibility
Gold nanoparticles are chemically inert and broadly compatible with biological molecules and assay platforms. They can be integrated with proteins, nucleic acids, polymers, and optical or electrochemical interfaces without interfering with biological recognition processes.
Package Contents
Each unit contains:
• 500 mL of 50 nm citrate-stabilized gold nanoparticle colloidal suspension
• Optical characterization and quality control documentation
Required Materials (Not Provided)
• UV–Vis spectrophotometer
• Dynamic Light Scattering (DLS) instrument (recommended)
• Standard laboratory glassware and pipettes
• Buffers compatible with downstream functionalization
Sample Handling & Use
• Gently swirl before use to ensure uniform suspension.
• Avoid vigorous shaking or sonication that may induce aggregation.
• Dilute using compatible aqueous buffers if required.
• Minimize repeated contamination of the stock solution.
Quality Control
• Each production lot is characterized for SPR peak position, particle concentration, and size distribution.
• Batch-to-batch consistency supports reproducibility for biodevice development workflows.
• Optical density and size metrics follow standardized QC procedures.
Storage & Stability
• Store at 4–25 °C.
• Do not freeze.
• Protect from prolonged exposure to direct light.
• Proper storage maintains colloidal stability and optical performance throughout shelf life.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic use.
• Performance may vary depending on buffer composition and functionalization strategy.
Applications
• Biosensor and plasmonic device development
• Optical and colorimetric assay design
• Protein and antibody conjugate preparation
• Microfluidic and lab-on-chip systems
• Biodevice calibration and benchmarking
• Imaging and analytical probing
