Product Description
The BioDeviceLab 10 nm Gold Nanoparticles are monodisperse colloidal gold particles engineered for consistent optical performance and surface reactivity. At ~10 nm diameter, these nanoparticles exhibit a strong localized surface plasmon resonance (LSPR) peak in the visible spectrum, facilitating robust UV–Vis–based detection, colorimetric assay design, and plasmonic sensing.
Manufactured using controlled synthesis and stabilization with citrate/tannic acid ligands, these gold nanoparticles have narrow size distributions (<15% dispersity) and high batch-to-batch reproducibility. The aqueous colloidal formulation supports compatibility with biological assays, microfluidic platforms, and biosensor development pipelines.
The characteristic LSPR absorbance peak near 520–525 nm enables both qualitative and quantitative optical readouts, while the high surface area promotes effective passive adsorption of proteins and ligands. The 500 mL volume supports large-scale conjugate preparation, assay calibration, and iterative experimental use.
Intended Use
This product is intended for research applications including:
• Biosensor and optical assay development
• Passive conjugation to proteins, antibodies, and ligands
• Signal enhancement in colorimetric and plasmonic systems
• Microfluidic and lab-on-chip integration
• Biodevice calibration and benchmarking
• Imaging contrast and analytical characterization
Principle of Operation
Gold nanoparticles exhibit localized surface plasmon resonance (LSPR), where conduction electrons oscillate collectively in response to incident light.
• The LSPR peak position and intensity depend on particle size, shape, and surrounding medium.
• Biomolecule binding or environmental shifts alter optical signals, enabling sensing modalities.
• High surface reactivity facilitates non-covalent adsorption and functionalization.
• These properties make 10 nm gold nanoparticles effective reporters and signal amplifiers in diverse biosensing platforms.
Calibration & Optical Characterization
• Optical density (OD) is defined at the SPR peak using a 1 cm pathlength.
• UV–Vis spectroscopy is used to verify the characteristic absorbance peak (~520–525 nm).
• Particle concentration and molar extinction coefficients are calculated at OD = 1.
• Dynamic Light Scattering (DLS) confirms hydrodynamic size and dispersity.
• Optical and size metrics should be verified in each experimental workflow.
Analytical & Physical Characteristics
Nominal particle diameter: ~10 nm
Peak SPR wavelength: ~520–525 nm
Particle concentration: ~2.70 × 10¹³ particles/mL
Weight concentration: ~1.50 × 10⁻¹ mg/mL
Molar extinction coefficient: ~2.60 × 10⁷ M⁻¹·cm⁻¹
Size dispersity: <15%
Surface area per particle: ~3.14 × 10² nm²
Surface-to-volume ratio: ~0.6
Particle mass: ~1.02 × 10⁻¹⁷ g
Molar mass: ~4.88 × 10⁶ g/mol
Molar concentration: ~2.20 × 10⁻⁸ M
Supplied volume: 500 mL
Selectivity & Compatibility
Gold nanoparticles are chemically inert and broadly compatible with biological molecules and assay platforms. They can be integrated with proteins, nucleic acids, polymers, and electrochemical interfaces without interfering with recognition or sensing performance.
Package Contents
Each unit contains:
• 500 mL of 10 nm citrate-stabilized gold nanoparticle colloidal suspension
• Optical characterization and quality control documentation
Required Materials (Not Provided)
• UV–Vis spectrophotometer
• Dynamic Light Scattering (DLS) instrument (recommended)
• Standard pipettes and laboratory consumables
• Buffers compatible with downstream functionalization
Sample Handling & Use
• Swirl gently before use to ensure uniform suspension.
• Avoid harsh agitation or sonication that may induce aggregation.
• Dilute with compatible aqueous buffers for downstream applications.
• Minimize repeated contamination of the stock solution.
Quality Control
• Each production lot is verified for SPR peak position, particle concentration, and size distribution.
• Batch consistency supports reproducibility for biodevice development workflows.
• Optical density and size metrics follow standardized QC practices.
Storage & Stability
• Store at 4–25 °C.
• Do not freeze.
• Protect from prolonged direct light exposure.
• Proper storage maintains colloidal stability and performance over the product’s shelf life.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic use.
• Performance may vary with buffer conditions and functionalization strategies.
Applications
• Biosensor and plasmonic device development
• Optical assay design and enhancement
• Protein and antibody conjugate preparation
• Microfluidic and lab-on-chip systems
• Biodevice calibration and benchmarking
• Imaging contrast and analytical probing
