Product Description
The BioDeviceLab Human Interleukin-8 (IL-8 / CXCL8) ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed for the quantitative determination of IL-8 in biological samples. IL-8 is a pro-inflammatory chemokine secreted by intestinal epithelial cells, immune cells, and endothelial cells in response to microbial products, cytokines, and cellular stress.
IL-8 plays a central role in neutrophil recruitment, epithelial immune signaling, and amplification of inflammatory responses in the gut. Elevated IL-8 levels are observed in inflammatory bowel disease, infection, dysbiosis, and epithelial barrier disruption. In gut-on-chip platforms, IL-8 serves as a robust and dynamic biomarker for quantifying epithelial inflammatory activation under microbial challenge, cytokine stimulation, drug exposure, or barrier injury. This assay combines high analytical sensitivity, strong specificity, and robust reproducibility, making it suitable for both mechanistic inflammation studies and advanced biodevice validation workflows.
Intended Use
This kit is intended for the quantitative determination of human IL-8 in:
• Serum
• Plasma
• Cell culture supernatants
• Tissue culture media
Applications include gut-on-chip inflammatory response modeling, host–microbiome interaction studies, epithelial immune signaling analysis, inflammatory bowel disease research, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format:
• IL-8 present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody specific to IL-8 binds to the captured protein.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to the IL-8 concentration in the sample.
Calibration Curve
• A calibration curve is generated using recombinant human IL-8 standards with known concentrations.
• Mean absorbance values are plotted against IL-8 concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
• Sample concentrations are calculated by interpolation from the standard curve.
Analytical Performance
Dynamic detection range: 5 – 1,000 pg/mL
Limit of detection (LOD): ≤ 2 pg/mL
Intra-assay CV: < 10%
Inter-assay CV: < 8%
Selectivity & Specificity
The assay demonstrates high specificity for IL-8 with minimal cross-reactivity to other cytokines and chemokines (IL-6, IL-1β, TNF-α, MCP-1) (< 1%).
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• Recombinant human IL-8 standards
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Required Materials (Not Provided)
• Microplate reader capable of measuring absorbance at 450 nm
• Adjustable micropipettes and disposable tips
• Plate washer or manual washing equipment
• Plate shaker (recommended)
• Deionized or distilled water
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Avoid repeated freeze–thaw cycles.
• Samples exceeding the highest standard should be diluted using assay buffer.
• Particulate matter should be removed prior to analysis.
Quality Control
• Standards and controls should be included in each assay run.
• Duplicate measurements are recommended.
• Acceptance criteria should be established according to experimental requirements.
BioDeviceLab applies quality-controlled reagent preparation and batch-to-batch consistency practices to support reproducibility and scalability for biodevice development.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
• Opened reagents should be handled according to protocol recommendations.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic procedures.
• Results should be interpreted within the context of experimental design and inflammatory model.
Applications
• Gut-on-chip epithelial inflammatory response validation
• Host–microbiome immune crosstalk studies
• Inflammatory bowel disease modeling
• Drug and biologic screening
