The BioDeviceLab Human Ubiquitin C-Terminal Hydrolase L1 (UCH-L1) ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed for the quantitative determination of UCH-L1 in biological samples. UCH-L1 is a neuron-enriched deubiquitinating enzyme involved in the ubiquitin–proteasome pathway and is widely used as a biomarker of neuronal cell body injury and neuroaxonal damage.
Elevated UCH-L1 concentrations have been reported in traumatic brain injury (TBI), concussion, stroke, hypoxic–ischemic injury, and other neurological conditions involving acute neuronal injury. Quantification of UCH-L1 is valuable for investigating injury mechanisms, assessing injury severity, monitoring recovery trajectories, and evaluating therapeutic interventions. This assay combines high analytical sensitivity, strong specificity, and robust reproducibility, making it suitable for both routine laboratory research and advanced biodevice validation workflows.
Intended Use
This kit is intended for the quantitative determination of human UCH-L1 in:
• Serum
• Plasma
• Cerebrospinal fluid (CSF)
• Cell culture supernatants
Applications include traumatic brain injury and concussion research, stroke studies, neurotoxicity and neuroinflammation studies, organ-on-chip brain models, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format:
• UCH-L1 present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody specific to UCH-L1 binds to the captured protein.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to the UCH-L1 concentration in the sample.
Calibration Curve
• A calibration curve is generated using recombinant human UCH-L1 standards with known concentrations.
• Mean absorbance values are plotted against UCH-L1 concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
Analytical Performance
Dynamic detection range: 0.02 – 20 ng/mL
Limit of detection (LOD): ≤ 0.01 ng/mL
Intra-assay CV: < 10%
Inter-assay CV: < 12%
(Performance aligned with commercial UCH-L1 ELISA kits used in neurological biomarker research.)
Selectivity & Specificity
The assay demonstrates high specificity for UCH-L1 with minimal cross-reactivity. Representative specificity profile:
• UCH-L1: 100%
• UCH-L3 and other deubiquitinases: < 1%
• GFAP, Tau, S100B, neurofilament proteins: < 1%
This specificity supports reliable UCH-L1 quantification in complex biological matrices.
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• Recombinant human UCH-L1 standards
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Required Materials (Not Provided)
• Microplate reader capable of measuring absorbance at 450 nm
• Adjustable micropipettes and disposable tips
• Plate washer or manual washing equipment
• Plate shaker (recommended)
• Deionized or distilled water
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Avoid repeated freeze–thaw cycles.
• Samples exceeding the highest standard should be diluted using assay buffer.
• Hemolyzed or lipemic samples may affect assay accuracy.
Quality Control
• Standards and controls should be included in each assay run.
• Duplicate measurements are recommended.
• Acceptance criteria should be established according to experimental requirements.
BioDeviceLab applies quality-controlled reagent preparation and batch-to-batch consistency practices to support reproducibility and scalability for biodevice development.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
• Opened reagents should be handled according to protocol recommendations.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic procedures.
• Results should be interpreted within the context of experimental design and neurological condition.
Applications
• Traumatic brain injury and concussion research
• Stroke and hypoxic–ischemic injury studies
• Neurotoxicity and neuroaxonal damage assessment
• Brain-on-chip and neurovascular models
• Biodevice calibration and benchmarking
