Product Description
The BioDeviceLab Human S100 Calcium-Binding Protein B (S100B) ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed for the quantitative determination of S100B in biological samples. S100B is a calcium-binding protein predominantly expressed by astrocytes and Schwann cells and is widely used as a biomarker of blood–brain barrier disruption, astroglial activation, and neuronal injury.
Elevated levels of S100B are associated with traumatic brain injury (TBI), stroke, hypoxic–ischemic injury, neuroinflammation, and neurodegenerative disorders. Quantification of S100B is extensively used in neurological research to assess injury severity, monitor disease progression, and evaluate therapeutic response. This assay combines high analytical sensitivity, excellent specificity, and robust reproducibility, making it suitable for both routine laboratory research and advanced biodevice validation workflows.
Intended Use
This kit is intended for the quantitative determination of human S100B in:
• Serum
• Plasma
• Cerebrospinal fluid (CSF)
• Cell culture supernatants
Applications include brain injury and concussion research, stroke studies, neuroinflammation and neurodegeneration research, organ-on-chip brain models, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format:
• S100B present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody specific to S100B binds to the captured protein.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to the S100B concentration in the sample.
Calibration Curve
• A calibration curve is generated using recombinant human S100B standards with known concentrations.
• Mean absorbance values are plotted against S100B concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
• Sample concentrations are calculated by interpolation from the standard curve.
Analytical Performance
Dynamic detection range: 0.02 – 10 ng/mL
Limit of detection (LOD): ≤ 0.01 ng/mL
Intra-assay CV: < 10%
Inter-assay CV: < 12%
(Performance aligned with commercial S100B ELISA kits used in neurological biomarker research.)
Selectivity & Specificity
The assay demonstrates high specificity for S100B with minimal cross-reactivity. Representative specificity profile:
• S100B: 100%
• S100A1, S100A6, S100A8/A9: < 1%
• GFAP, Tau, Neurofilament proteins: < 1%
This specificity ensures accurate S100B quantification in complex biological matrices.
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• Recombinant human S100B standards
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Required Materials (Not Provided)
• Microplate reader capable of measuring absorbance at 450 nm
• Adjustable micropipettes and disposable tips
• Plate washer or manual washing equipment
• Plate shaker (recommended)
• Deionized or distilled water
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Avoid repeated freeze–thaw cycles.
• Samples exceeding the highest standard should be diluted using assay buffer.
• Hemolyzed or lipemic samples may affect assay accuracy.
Quality Control
• Standards and controls should be included in each assay run.
• Duplicate measurements are recommended.
• Acceptance criteria should be established according to experimental requirements.
BioDeviceLab applies quality-controlled reagent preparation and batch-to-batch consistency practices to support reproducibility and scalability for biodevice development.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
• Opened reagents should be handled according to protocol recommendations.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic procedures.
• Results should be interpreted within the context of experimental design and neurological condition.
Applications
• Traumatic brain injury and concussion research
• Stroke and hypoxic–ischemic injury studies
• Neuroinflammation and neurodegeneration research
• Brain-on-chip and neurovascular models
• Biodevice calibration and benchmarking
