Product Description
The BioDeviceLab Human Amyloid Precursor Protein (APP) ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed for the quantitative determination of full-length APP in biological samples. APP is a transmembrane glycoprotein widely expressed in neuronal and non-neuronal tissues and serves as the precursor for amyloid-β peptides implicated in Alzheimer’s disease and other neurodegenerative disorders.
Quantification of APP is essential for understanding amyloidogenic and non-amyloidogenic processing pathways, evaluating disease mechanisms, and assessing therapeutic interventions targeting APP metabolism. This assay combines high analytical sensitivity, excellent specificity, and robust reproducibility, making it suitable for both routine laboratory research and advanced biodevice validation workflows.
Intended Use
This kit is intended for the quantitative determination of human APP in:
• Serum
• Plasma
• Cerebrospinal fluid (CSF)
• Cell culture supernatants
Applications include Alzheimer’s disease research, neurodegeneration studies, drug discovery and screening, organ-on-chip models, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format:
• APP present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody recognizing a distinct APP epitope binds to the captured protein.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to the APP concentration in the sample.
Calibration Curve
• A calibration curve is generated using recombinant human APP standards with known concentrations.
• Mean absorbance values are plotted against APP concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
• Sample concentrations are calculated by interpolation from the standard curve.
Analytical Performance
Dynamic detection range: 0.3 – 50 ng/mL
Limit of detection (LOD): ≤ 0.2 ng/mL
Intra-assay CV: < 10%
Inter-assay CV: < 12%
(Performance aligned with commercial APP ELISA kits used in neurodegeneration research.)
Selectivity & Specificity
The assay demonstrates high specificity for human APP with minimal cross-reactivity. Representative specificity profile:
• Full-length human APP: 100%
• Amyloid-β peptides (Aβ40, Aβ42): minimal cross-reactivity
• Tau, GFAP, neurofilament proteins: < 1%
This specificity enables selective quantification of APP independent of downstream amyloid cleavage products.
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• Recombinant human APP standards
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Required Materials (Not Provided)
• Microplate reader capable of measuring absorbance at 450 nm
• Adjustable micropipettes and disposable tips
• Plate washer or manual washing equipment
• Plate shaker (recommended)
• Deionized or distilled water
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Avoid repeated freeze–thaw cycles.
• Samples exceeding the highest standard should be diluted using assay buffer.
• Hemolyzed or lipemic samples may affect assay accuracy.
Quality Control
• Standards and controls should be included in each assay run.
• Duplicate measurements are recommended.
• Acceptance criteria should be defined according to experimental requirements.
BioDeviceLab applies quality-controlled reagent preparation and batch-to-batch consistency practices to support reproducibility and scalability for biodevice development.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
• Opened reagents should be handled according to protocol recommendations.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic procedures.
• Results should be interpreted within the context of experimental design and sample matrix.
Applications
• Alzheimer’s disease and dementia research
• APP processing and amyloid pathway studies
• Drug discovery and therapeutic screening
• Organ-on-chip and neuronal tissue models
• Biodevice calibration and benchmarking
