Product Overview
The BioDeviceLab Human IL-23 ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed to quantify IL-23, a heterodimeric cytokine composed of p19 and p40 subunits and produced primarily by dendritic cells and macrophages following microbial sensing. IL-23 is a central driver of Th17 cell maintenance and expansion and promotes downstream cytokines such as IL-17A, IL-22, and GM-CSF.
In gastrointestinal biology, IL-23 plays a pivotal role in mucosal inflammation, epithelial barrier modulation, and host–microbiome immune balance. Dysregulated IL-23 signaling is strongly implicated in inflammatory bowel disease and chronic intestinal inflammation. In gut-on-chip platforms, IL-23 serves as a critical biomarker linking innate microbial sensing to adaptive Th17 responses and epithelial stress signaling. This assay combines high analytical sensitivity, strong specificity for the IL-23 heterodimer, and reproducible performance suitable for mechanistic studies and biodevice validation workflows.
Intended Use
This kit is intended for the quantitative determination of IL-23 in:
• Serum
• Plasma
• Cell culture supernatants
Applications include gut-on-chip Th17 pathway studies, dendritic cell and macrophage activation assays, host–microbiome immune interaction research, inflammation and infection models, drug and biologic screening, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format specific to IL-23:
• IL-23 present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody recognizing a distinct epitope on IL-23 binds the captured cytokine.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to IL-23 concentration.
Calibration Curve
• Generated using recombinant human IL-23 standards.
• Mean absorbance values plotted versus concentration on a semi-logarithmic scale.
• Four-parameter logistic (4PL) regression recommended.
• Sample concentrations interpolated from the standard curve.
Analytical Performance
Dynamic Detection Range
• 10 – 2,000 pg/mL
Analytical Sensitivity
• Limit of Detection (LOD): ≤ 5 pg/mL
Precision
• Intra-assay CV: < 10%
• Inter-assay CV: < 8%
Selectivity & Specificity
High specificity for IL-23 with minimal cross-reactivity to IL-12p70, IL-12p40, IL-17A, and IL-22 (< 1%).
Kit Components
• Antibody-coated 96-well microplate
• IL-23 standards (lyophilized)
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed protocol
Applications
• Th17 immune axis profiling
• Gut-on-chip epithelial–immune signaling
• Host–microbiome interaction studies
• Chronic intestinal inflammation models
• Drug and biologic screening
• Biodevice calibration and benchmarking
