Product Description
Occludin, encoded by the OCLN gene, is an integral membrane protein with an approximate molecular weight of 60–65 kDa and is a core structural component of tight junctions. Occludin spans the plasma membrane multiple times and contributes directly to paracellular barrier regulation, junctional stability, and epithelial and endothelial permeability control. Its expression and localization are closely associated with barrier integrity and tissue homeostasis.
Occludin is localized at cell–cell contacts within tight junction complexes, where it interacts with scaffold proteins such as ZO-1, ZO-2, and ZO-3, as well as the actin cytoskeleton. Immunostaining produces a characteristic continuous or segmented junctional pattern that provides a sensitive readout of tight junction organization, maturation, and remodeling under physiological or perturbed conditions.
The BioDeviceLab Occludin Antibody is supplied as a purified monoclonal antibody to support flexible assay design. It is compatible with fixed 2D cultures, 3D tissue models, organoids, tissue sections, and microfluidic or biodevice-integrated platforms. Occludin antibodies are widely used in barrier biology, epithelial research, tissue engineering, and translational biodevice validation.
Intended Use
This product is intended for research applications including
• Tight junction visualization and barrier integrity assessment
• Epithelial and endothelial permeability studies
• Immunofluorescence and immunocytochemistry imaging
• Organoid, spheroid, and 3D culture characterization
• Cell–cell junction analysis in mixed or co-culture systems
• Tissue engineering and regenerative medicine research
• Biosensor, biodevice, and assay biological validation
• Studies of junctional remodeling and epithelial organization
Principle of Operation
Occludin (OCLN) is localized to tight junctions at epithelial and endothelial cell–cell interfaces. Binding of the antibody following permeabilization enables
• Spatial visualization of tight junction architecture by immunofluorescence
• Assessment of junctional continuity, alignment, and barrier integrity
• Integration into multiplex assays with scaffold, cytoskeletal, or nuclear markers
Technical Information
Target antigen: Occludin (OCLN)
Alternative names: Tight junction protein occludin
Protein type: Multi-pass transmembrane tight junction protein
Approximate molecular weight: ~60–65 kDa
Cellular localization: Cell surface at tight junctions
Clonality: Monoclonal
Host species: Mouse
Isotype: IgG (clone dependent)
Commonly used commercial clones: OC-3F10, Z-T22, 19-1B
Validated applications (clone dependent): IF/ICC, immunohistochemistry, western blot
Typical stock concentration (industry reference): approximately 0.5 mg/mL
Reactivity: Human (other species dependent on clone validation)
Sample Volume and Typical Usage
Recommended sample volumes depend on assay format and platform and should be optimized experimentally. Common working ranges are summarized below.
Immunofluorescence / Immunocytochemistry
• Typical staining volume: 50–200 µL per sample
• Applicable to coverslips, chamber slides, well plates, tissue sections, and on-chip staining chambers
• Samples must be permeabilized to enable intracellular access
Organoid and 3D Culture Staining
• Typical staining volume: 100–500 µL per sample
• Volume depends on matrix composition, organoid density, and vessel geometry
• Extended incubation times may be required to ensure junctional labeling
Microfluidic and Biodevice Platforms
• Typical staining volume: 10–100 µL per device
• Volume depends on channel dimensions and surface area
• Permeabilization conditions should be optimized to preserve junctional structure
General notes
• Antibody concentration is more critical than absolute volume
• Maintain consistent antibody-to-surface ratios
• Optimization is recommended for each assay format and platform
Selectivity and Compatibility
Occludin is selectively localized at tight junctions and provides a direct readout of epithelial and endothelial barrier integrity. The antibody is compatible with standard fixation and permeabilization protocols and performs reliably in conventional culture systems, tissue sections, and microfluidic or biodevice-integrated workflows.
Package Contents
Each unit contains
• Purified BioDeviceLab Occludin (OCLN) monoclonal antibody
• Lot-specific quality control documentation
Required Materials
• Fluorescent secondary antibody for unconjugated formats
• Fixation and permeabilization buffers
• Blocking and wash buffers compatible with IF
• Fluorescence microscope
• Optional isotype and negative controls
Sample Handling and Use
• Store according to label instructions
• Avoid repeated freeze–thaw cycles
• Optimize antibody concentration, volume, and permeabilization conditions
• Use appropriate controls for comparative analysis
Quality Control
Each lot is evaluated for binding specificity and junctional staining performance. The antibody is designed to support reproducible tight junction visualization across diverse experimental platforms and longitudinal studies.
Storage and Stability
• Store at 2–8 °C
• Do not freeze under standard storage conditions
• Protect from contamination and prolonged light exposure
• Proper storage preserves antibody stability and performance
Applications
• Tight junction and barrier biology
• Epithelial and endothelial research
• Tissue engineering and regenerative medicine
• Organoid and 3D culture systems
• Immunofluorescence-based junctional analysis
• Biosensing and biodevice validation
• Cell polarity and junctional remodeling studies
