Product Description
Zonula Occludens-1 (ZO-1), also known as Tight Junction Protein 1 (TJP1), is a cytoplasmic scaffold protein with an approximate molecular weight of 220 kDa that plays a central role in the assembly, maintenance, and regulation of tight junctions in epithelial and endothelial cells. ZO-1 interacts with transmembrane tight junction proteins, including claudins and occludin, and links them to the actin cytoskeleton, thereby coordinating barrier integrity and cell polarity.
ZO-1 is localized at cell–cell junctions and is widely used as a canonical marker of tight junction formation, epithelial organization, and barrier maturation. Because ZO-1 is an intracellular protein, antibody detection requires membrane permeabilization. ZO-1 staining produces a characteristic continuous or punctate junctional pattern that is highly informative for assessing junctional continuity, organization, and remodeling under physiological or perturbed conditions.
The BioDeviceLab ZO-1 Antibody is supplied as a purified monoclonal antibody to support flexible assay design. It is optimized for immunofluorescence and immunocytochemistry workflows and is compatible with fixed 2D cultures, 3D models, tissue sections, and device-integrated systems. ZO-1 antibodies are routinely used in epithelial biology, barrier research, tissue engineering, and translational biodevice validation.
Intended Use
This product is intended for research applications including
• Tight junction visualization and characterization
• Epithelial and endothelial barrier assessment
• Immunofluorescence and immunocytochemistry imaging
• Organoid, spheroid, and 3D culture characterization
• Cell–cell junction analysis in mixed or co-culture systems
• Tissue engineering and regenerative medicine research
• Biosensor, biodevice, and assay biological validation
• Studies of junctional remodeling and cell polarity
Principle of Operation
ZO-1 (TJP1) is localized to the cytoplasmic face of tight junctions at cell–cell interfaces. Binding of the antibody following permeabilization enables
• Spatial visualization of tight junction architecture by immunofluorescence
• Assessment of junctional continuity, alignment, and integrity
• Integration into multiplex assays with membrane, cytoskeletal, or nuclear markers
Technical Information
Target antigen: Zonula Occludens-1 (ZO-1), Tight Junction Protein 1
Gene name: TJP1
Protein type: Cytoplasmic tight junction scaffold protein
Approximate molecular weight: ~220 kDa
Cellular localization: Cell–cell junctions, cytoplasmic face of tight junctions
Clonality: Monoclonal
Host species: Mouse
Isotype: IgG (clone dependent)
Commonly used commercial clones: ZO1-1A12, 1A12, R40.76 (clone dependent)
Validated applications (clone dependent): IF/ICC, immunohistochemistry, western blot
Typical stock concentration (industry reference): approximately 0.5 mg/mL
Reactivity: Human (other species dependent on clone validation)
Sample Volume and Typical Usage
Recommended sample volumes depend on assay format and platform and should be optimized experimentally. Common working ranges are summarized below.
Immunofluorescence / Immunocytochemistry
• Typical staining volume: 50–200 µL per sample
• Applicable to coverslips, chamber slides, well plates, tissue sections, and on-chip staining chambers
• Samples must be permeabilized to enable intracellular access
Organoid and 3D Culture Staining
• Typical staining volume: 100–500 µL per sample
• Volume depends on matrix composition, organoid density, and vessel geometry
• Extended incubation times are often required for uniform junctional labeling
Microfluidic and Biodevice Platforms
• Typical staining volume: 10–100 µL per device
• Volume depends on channel dimensions and surface area
• Permeabilization conditions should be optimized to preserve junctional structure
General notes
• Antibody concentration is more critical than absolute volume
• Maintain consistent antibody-to-surface ratios
• Optimization is recommended for each platform and fixation method
Selectivity and Compatibility
ZO-1 is selectively localized at tight junctions and provides a robust readout of junctional organization and epithelial or endothelial barrier integrity. The antibody is compatible with standard fixation and permeabilization protocols and performs reliably in conventional culture systems, tissue sections, and microfluidic or biodevice-integrated workflows.
Package Contents
Each unit contains
• Purified BioDeviceLab ZO-1 (TJP1) monoclonal antibody
• Lot-specific quality control documentation
Required Materials
• Fluorescent secondary antibody for unconjugated formats
• Fixation and permeabilization buffers
• Blocking and wash buffers compatible with IF
• Fluorescence microscope
• Optional isotype and negative controls
Sample Handling and Use
• Store according to label instructions
• Avoid repeated freeze–thaw cycles
• Optimize antibody concentration, volume, and permeabilization conditions
• Use appropriate controls for comparative analysis
Quality Control
Each lot is evaluated for binding specificity and junctional staining performance. The antibody is designed to support reproducible tight junction visualization across diverse experimental platforms and longitudinal studies.
Storage and Stability
• Store at 2–8 °C
• Do not freeze under standard storage conditions
• Protect from contamination and prolonged light exposure
• Proper storage preserves antibody stability and performance
Applications
• Tight junction and barrier biology
• Epithelial and endothelial research
• Tissue engineering and regenerative medicine
• Organoid and 3D culture systems
• Immunofluorescence-based junctional analysis
• Biosensing and biodevice validation
• Cell polarity and junctional remodeling studies
