Product Overview
The BioDeviceLab Human α-Synuclein ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed to quantify α-synuclein, a presynaptic neuronal protein critically involved in synaptic vesicle trafficking and neurotransmitter release. Dysregulation, aggregation, or abnormal release of α-synuclein is a hallmark of synucleinopathies, including Parkinson’s disease, dementia with Lewy bodies, and multiple system atrophy.
Beyond classical neurodegeneration, α-synuclein is increasingly recognized as a key mediator in the gut–brain axis. It is expressed in enteric neurons and can respond to microbial products, inflammation, and epithelial barrier disruption, making it a powerful biomarker for studying microbiome-driven neuroinflammatory signaling. In gut-on-chip and neuro-on-chip systems, α-synuclein serves as a sensitive readout of synaptic stress, inflammatory crosstalk, and neurotoxic insults. This assay combines high analytical sensitivity, strong specificity, and robust reproducibility suitable for mechanistic studies, drug screening, and biodevice validation.
Intended Use
This kit is intended for the quantitative determination of total α-synuclein in:
• Serum
• Plasma
• Cerebrospinal fluid (CSF)
• Cell culture supernatants
Applications include neurodegeneration and synucleinopathy research, gut–brain axis modeling, neuroinflammation studies, neuro-on-chip and organoid platforms, drug and biologic screening, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format:
• α-Synuclein present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody recognizing a distinct α-synuclein epitope binds to the captured protein.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to α-synuclein concentration.
Calibration Curve
• Generated using recombinant human α-synuclein standards.
• Mean absorbance values plotted versus concentration on a semi-logarithmic scale.
• Four-parameter logistic (4PL) regression recommended.
• Sample concentrations interpolated from the standard curve.
Analytical Performance
Dynamic Detection Range
• 40 – 10,000 pg/mL
Analytical Sensitivity
• Limit of Detection (LOD): ≤ 25 pg/mL
Precision
• Intra-assay CV: < 8%
• Inter-assay CV: < 10%
Selectivity & Specificity
High specificity for human α-synuclein with minimal cross-reactivity to Tau, β-amyloid, GFAP, NfL, or UCH-L1 (< 1%).
Applications
• Synaptic dysfunction and neurodegeneration studies
• Parkinson’s disease and synucleinopathy research
• Gut–brain axis and microbiome-driven neuroinflammation
• Neuro-on-chip and enteric nervous system models
• Drug and biologic screening
• Biodevice calibration and benchmarking
