Product Description
The BioDeviceLab 10 nm Endotoxin-Free Gold Nanoparticles are precision-engineered colloidal particles designed to meet the needs of sensitive biological and analytical applications. At ~10 nm diameter, these nanoparticles exhibit a pronounced localized surface plasmon resonance (LSPR) in the visible spectrum, enabling robust UV–Vis–based detection, colorimetric assay design, and plasmonic sensing. The endotoxin-free grade supports applications that interface with delicate cell cultures or immunological systems sensitive to lipopolysaccharide (LPS) contamination.
Formulated with citrate/tannic acid stabilization in aqueous buffer and subjected to rigorous endotoxin removal procedures, these nanoparticles maintain narrow size distributions (<15% dispersity) and strong colloidal stability while reducing background immunostimulation in biologically sensitive assays.
The characteristic SPR absorbance peak near 520–525 nm enables reliable optical and spectroscopic characterization. The 500 mL bulk volume supports large-scale assay development, conjugate preparation, and iterative experimental use with reproducible quality across biodevice validation and translational research workflows.
Intended Use
This product is intended for research applications including:
• Endotoxin-sensitive biosensor development
• Cell-based assays and live-cell imaging
• Immune and cytokine research workflows
• Passive conjugation of proteins, antibodies, and ligands
• Optical and plasmonic signal amplification
• Microfluidic and lab-on-chip integration
Principle of Operation
Gold nanoparticles exhibit localized surface plasmon resonance (LSPR), where conduction electrons on the particle surface oscillate collectively in response to incident light.
• The SPR peak position and intensity are influenced by particle size, shape, and local environment.
• Biomolecule binding or environmental changes modulate optical signals, enabling sensing.
• High surface area at 10 nm supports effective biomolecule adsorption and functionalization.
• Endotoxin-free surface minimizes interference with sensitive biological systems.
Calibration & Optical Characterization
• Optical density (OD) is defined at the SPR peak wavelength using a 1 cm pathlength.
• UV–Vis spectroscopy is used to verify the characteristic absorbance peak (~520–525 nm).
• Particle concentration and molar extinction coefficients are calculated based on OD = 1.
• Dynamic Light Scattering (DLS) confirms hydrodynamic size and dispersity.
• Endotoxin assays (e.g., LAL) verify low LPS content for biologically sensitive applications.
Analytical & Physical Characteristics
Nominal particle diameter: ~10 nm
Peak SPR wavelength: ~520–525 nm
Particle concentration: ~2.7 × 10¹³ particles/mL
Weight concentration: ~1.5 × 10⁻¹ mg/mL
Molar extinction coefficient: ~2.6 × 10⁷ M⁻¹·cm⁻¹
Size dispersity: <15%
Surface area per particle: ~3.14 × 10² nm²
Surface-to-volume ratio: ~0.6
Particle mass: ~1.02 × 10⁻¹⁷ g
Molar mass: ~4.88 × 10⁶ g/mol
Molar concentration: ~2.20 × 10⁻⁸ M
Supplied volume: 500 mL
Endotoxin level: Certified low endotoxin (LPS) (specific specification available on request)
Selectivity & Compatibility
Gold nanoparticles are chemically inert and exhibit broad compatibility with biological molecules and assay platforms. Endotoxin-free formulation minimizes unintended activation of immune cells or cytokine pathways in cell-based assays, immunogenicity studies, and host–biomicroenvironment models.
Package Contents
Each unit contains:
• 500 mL of 10 nm endotoxin-free citrate-stabilized gold nanoparticle colloidal suspension
• Optical characterization and quality control documentation
• Endotoxin assurance report (e.g., LPS quantification certificate)
Required Materials (Not Provided)
• UV–Vis spectrophotometer
• DLS instrument (recommended)
• Standard laboratory glassware and pipettes
• Buffers suitable for downstream biological applications
Sample Handling & Use
• Gently swirl before use to ensure uniform suspension.
• Avoid harsh agitation that may induce aggregation.
• Dilute with compatible buffers for downstream applications.
• Use endotoxin-free consumables and reagents when conducting endotoxin-sensitive assays.
Quality Control
• Each production lot is verified for optical features, particle concentration, size distribution, and endotoxin level.
• Batch consistency supports reproducibility in sensitive biological workflows.
• Optical density, size, and endotoxin results follow standardized QC practices.
Storage & Stability
• Store at 4–25 °C.
• Do not freeze.
• Protect from prolonged direct light exposure.
• When stored properly, the colloid maintains stability and performance throughout the product’s shelf life.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic use.
• Performance may vary depending on buffer conditions and downstream functionalization strategy.
Applications
• Endotoxin-sensitive biosensor and plasmonic development
• Host–microbiome and immune activation modeling
• Cell culture and live-cell imaging
• Protein and antibody conjugate engineering
• Microfluidic and lab-on-chip systems
• Biodevice calibration and benchmarking
