Product Description
The BioDeviceLab Human Interleukin-17A (IL-17A) ELISA Kit is a high-performance enzyme-linked immunosorbent assay developed for the quantitative determination of IL-17A in biological samples. IL-17A is a pro-inflammatory cytokine predominantly produced by Th17 cells, γδ T cells, and innate lymphoid cells, and plays a central role in mucosal immunity, epithelial defense, and chronic inflammation.
In the gastrointestinal tract, IL-17A regulates epithelial antimicrobial responses, neutrophil recruitment, and barrier integrity. Dysregulated IL-17A signaling is strongly associated with inflammatory bowel disease, autoimmune disorders, infection-driven inflammation, and altered host–microbiome interactions. In gut-on-chip systems, IL-17A serves as a critical biomarker for Th17-mediated immune activation, epithelial–immune crosstalk, and evaluation of anti-inflammatory or immunomodulatory therapeutics. This assay combines high analytical sensitivity, excellent specificity, and robust reproducibility, making it suitable for mechanistic inflammation studies and advanced biodevice validation workflows.
Intended Use
This kit is intended for the quantitative determination of human IL-17A in:
• Serum
• Plasma
• Cell culture supernatants
• Tissue culture media
Applications include gut-on-chip Th17 inflammation modeling, mucosal immune response studies, inflammatory bowel disease research, host–microbiome interaction analysis, and biodevice calibration and benchmarking.
Assay Principle
This assay employs a sandwich ELISA format:
• IL-17A present in the sample binds to capture antibodies immobilized on a 96-well microplate.
• A biotinylated detection antibody specific to IL-17A binds to the captured cytokine.
• An enzyme conjugate is added, followed by chromogenic substrate (TMB).
• The enzymatic reaction generates a colorimetric signal measured at 450 nm.
• Absorbance is directly proportional to the IL-17A concentration in the sample.
Calibration Curve
• A calibration curve is generated using recombinant human IL-17A standards with known concentrations.
• Mean absorbance values are plotted against IL-17A concentration on a semi-logarithmic scale.
• A four-parameter logistic (4PL) regression model is recommended for curve fitting.
• Sample concentrations are calculated by interpolation from the standard curve.
Analytical Performance
Dynamic detection range: 5 – 1,000 pg/mL
Limit of detection (LOD): ≤ 2 pg/mL
Intra-assay CV: < 10%
Inter-assay CV: < 12%
Selectivity & Specificity
The assay demonstrates high specificity for IL-17A with minimal cross-reactivity to related cytokines (IL-17F, IL-22, IL-6, TNF-α) (< 1%).
Kit Components
Each kit contains sufficient reagents for up to 96 determinations:
• Antibody-coated 96-well microplate
• Recombinant human IL-17A standards
• Biotinylated detection antibody
• Enzyme conjugate
• Assay buffer
• Wash buffer (10×)
• TMB substrate
• Stop solution
• Detailed assay protocol
Required Materials (Not Provided)
• Microplate reader capable of measuring absorbance at 450 nm
• Adjustable micropipettes and disposable tips
• Plate washer or manual washing equipment
• Plate shaker (recommended)
• Deionized or distilled water
Sample Collection & Handling
• Collect samples using standard laboratory procedures.
• Avoid repeated freeze–thaw cycles.
• Samples exceeding the highest standard should be diluted using assay buffer.
• Clarify samples prior to analysis if particulate matter is present.
Quality Control
• Standards and controls should be included in each assay run.
• Duplicate measurements are recommended.
• Acceptance criteria should be established according to experimental requirements.
BioDeviceLab applies quality-controlled reagent preparation and batch-to-batch consistency practices to support reproducibility and scalability for biodevice development.
Storage & Stability
• Store all components at 2–8 °C.
• Do not freeze reagents unless explicitly stated.
• Unopened kits are stable until the stated expiration date.
• Opened reagents should be handled according to protocol recommendations.
Limitations
• For research use only.
• Not intended for diagnostic or therapeutic procedures.
• Results should be interpreted within the context of experimental design and Th17-mediated inflammation models.
Applications
• Gut-on-chip Th17 and mucosal inflammation studies
• Host–microbiome immune interaction research
• Inflammatory bowel disease modeling
• Drug and biologic screening
• Biodevice calibration and benchmarking
